Experience of monitoring shear movements in the overburden strata around longwall panels

Surface subsidence monitoring shows horizontal movements occur around longwall panels for a considerable distance outside the footprint of a longwall panel; typically several hundred metres to several kilometres. Less is known about how these movements are distributed between the surface and the mining horizon. A range of systems have been developed to measure how horizontal movements are distributed within the overburden strata generally and sometimes around specific geological structures. This paper describes the experience of using a range of these systems at various sites and some of the insights that these measurements bring with particular focus on the use of deep inclinometers. The capability to measure induced displacements has developed over time from surface observations to use of borehole systems such as multi-arm callipers, downhole camera imaging and specially installed inclinometers placed to depths up to 300 m. Some techniques such as open boreholes and the multi-arm, oriented calliper have mainly been used at shallow depths where breakout and squeezing ground do not compromise the measurements. Others such as the borehole camera provide context but are not so suitable for quantitative measurement. The inclinometer installed in a large diameter borehole backfilled with pea-gravel has been found to provide high resolution measurements up to a horizontal displacement on any one horizon of about 60-80 mm. Inclinometers have been used at multiple sites around Australia to measure shear displacements to depths of up to about 300m. Shaped array accelerometers are an alternative that provide temporal resolution of a few minutes and provide continuous monitoring over a limited interval but tend to be most useful for monitoring the onset of low magnitude shear displacements

Infrequent expression of the cancer-testis antigen, PASD1, in ovarian cancer

Ovarian cancer is very treatable in the early stages of disease; however, it is usually detected in the later stages, at which time, treatment is no longer as effective. If discovered early (Stage I), there is a 90% chance of five-year survival. Therefore, it is imperative that early-stage biomarkers are identified to enhance the early detection of ovarian cancer. Cancer-testis antigens (CTAs), such as Per ARNT SIM (PAS) domain containing 1 (PASD1), are unique in that their expression is restricted to immunologically restricted sites, such as the testis and placenta, which do not express MHC class I, and cancer, making them ideally positioned to act as targets for immunotherapy as well as potential biomarkers for cancer detection where expressed. We examined the expression of PASD1a and b in a number of cell lines, as well as eight healthy ovary samples, eight normal adjacent ovarian tissues, and 191 ovarian cancer tissues, which were predominantly stage I (n = 164) and stage II (n = 14) disease. We found that despite the positive staining of skin cancer, only one stage Ic ovarian cancer patient tissue expressed PASD1a and b at detectable levels. This may reflect the predominantly stage I ovarian cancer samples examined. To examine the restriction of PASD1 expression, we examined endometrial tissue arrays and found no expression in 30 malignant tumor tissues, 23 cases of hyperplasia, or 16 normal endometrial tissues. Our study suggests that the search for a single cancer-testes antigen/biomarker that can detect early ovarian cancer must continue

PASD1: a promising target for the immunotherapy of haematological malignancies

In general, there is a lack of good immunotherapy targets within the spectrum of haematological malignancies. However haematopoietic stem cell transplants and continuing antigen discovery have allowed further insight into how further improvements in outcomes for patients might be achieved. Most patients with haematological malignancies can be treated with conventional therapies such as radio- and chemotherapy and will attain first remission. However the removal of residual diseased cells is essential to prevent relapse and its associated high mortality. PASD1 is one of the most tissue restricted cancer-testis (CT) antigens with expression limited to primary spermatagonia in healthy tissue. However, characterisation of PASD1 expression in cancers has been predominantly focussed on haematological malignancies where the inappropriate expression of PASD1 was first identified. PASD1 has one of the highest frequencies of expression of all CT antigens in acute myeloid leukaemia, with some suggestion of its role as a biomarker in diffuse large B-cell lymphoma. Here we describe the characterisation of the function and expression patterns of PASD1 in cell lines and primary tissues. Development of DNA vaccines targeting PASD1 epitopes demonstrate effective ex vivo T-cell responses in terms of IFNγ secretion and tumour cell killing. Of particular note these vaccines have led to the destruction of cells which process and present endogenous PASD1 indicating that effectively primed CTLs could kill PASD1-positive tumour cells

Fostering Undergraduate Research Experiences In Management Information Systems Through The Research Group Framework

The purpose of this paper is to propose an innovative approach to engaged learning.  Founded on the principles of a scholarly think-tank and administered along the lines of a consulting organization, the proposed ‘Research Group’ framework is designed to facilitate effective and efficient undergraduate research experiences in Management Information Systems and other business areas.  In doing so, the program better prepares students for success in graduate school and their chosen careers.  In this paper, we introduce the research group framework and describe its four fundamental elements.  Examples of are then presented to illustrate both scholar-based and organization-based research experiences.  We conclude with a discussion on the challenges of developing a formal undergraduate research program

University of Maine Athletic Facilities Master Plan

The Harold Alfond Foundation’s historic investment in Maine and its people includes a $240 million commitment to the University of Maine System to bring transformative change to the state’s largest educational, research, innovation and talent development asset. Of that,$90 million will be used for athletic facilities at the University of Maine and the well-being of Maine people, providing support to maintain excellence in the state’s only Division I athletics program, strengthen gender equity, and provide a preferred destination for high school sports championships, large academic fairs and competitions, and community events. All of the university’s students and people from throughout Maine will be able to use the state-of-the-art athletic and convening venues at the state’s flagship university in Orono

New targets for therapy: antigen identification in adults with B-cell acute lymphoblastic leukaemia

Acute lymphoblastic leukaemia (ALL) in adults is a rare and difficult-to-treat cancer that is characterised by excess lymphoblasts in the bone marrow. Although many patients achieve remission with chemotherapy, relapse rates are high and the associated impact on survival devastating. Most patients receive chemotherapy and for those whose overall fitness supports it, the most effective treatment to date is allogeneic stem cell transplant that can improve overall survival rates in part due to a ‘graft-versus-leukaemia’ effect. However, due to the rarity of this disease, and the availability of mature B-cell antigens on the cell surface, few new cancer antigens have been identified in adult B-ALL that could act as targets to remove residual disease in first remission or provide alternative targets for escape variants if and when current immunotherapy strategies fail. We have used RT-PCR analysis, literature searches, antibody-specific profiling and gene expression microarray analysis to identify and prioritise antigens as novel targets for the treatment of adult B-ALL

Short-term exposure to carbon monoxide and myocardial infarction: A systematic review and meta-analysis

BACKGROUND: Previous studies suggest an association between short-term exposure to carbon monoxide and myocardial infarction. We performed a systematic review and meta-analysis to assess current evidence on this association to support the update of the World Health Organization (WHO) Global Air Quality Guidelines. METHODS: We searched Medline, Embase and Cochrane Central Register of Controlled Trials to update the evidence published in a previous systematic review up to 30th September 2018 for studies investigating the association between short-term exposure to ambient carbon monoxide (up to lag of seven days) and emergency department visits or hospital admissions and mortality due to myocardial infarction. Two reviewers assessed potentially eligible studies and performed data extraction independently. Random-effects meta-analysis was used to derive the pooled risk estimate per 1 mg/m3 increase in ambient carbon monoxide concentration. Risk of bias in individual studies was assessed using a domain-based assessment tool. The overall certainty of the body of evidence was evaluated using an adapted certainty of evidence assessment framework. RESULTS: We evaluated 1,038 articles from the previous review and our updated literature search, of which, 26 satisfied our inclusion criteria. Overall, myocardial infarction was associated with exposure to ambient carbon monoxide concentration (risk ratio of 1.052, 95% confidence interval 1.017-1.089 per 1 mg/m3 increase). A third of studies were assessed to be at high risk of bias (RoB) due to inadequate adjustment for confounding. Using an adaptation of the Grading of Recommendations Assessment, Development and Evaluation (GRADE) framework, the overall evidence was assessed to be of moderate certainty. CONCLUSIONS: This review demonstrated that the pooled risk ratio for myocardial infarction was 1.052 (95% CI 1.017-1.089) per 1 mg/m3 increase in ambient carbon monoxide concentration. However, very few studies originated from low- and middle-income countries

National Crystallography Service (NCS) Grid Service

Conference poster about the NCS Grid Service.The EPSRC funded National Crystallography Service (NCS) is a facility available to the entire UK academic Chemistry community. The EPSRC funds a team of experts and 'state of the art' instrumentation, based in Southampton University School of Chemistry, to provide this service. This is an exceptionally important service as crystal structure determination is easily the most information rich method of characterisation of a compound and many research papers cannot be published without confirmation of identity by crystal structure analysis